0199636567,Mutation Detection: A Practical Approach PA 188 Irl, Vol. 188,Mutation,Detection:,A,Practical,Approach,PA,188,Irl,,Vol.,188,buy,book,books,purchase,read,Forrest Cotton Edkins,Rand McNally Staff,R. G. Cotton,S. Forrest
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"Our expression and our words never coincide, which is why the animals don't understand us."

  - Chazal

 

 

Mutation Detection:
A Practical Approach PA 188 Irl, Vol. 188

 
  by Rand McNally Staff, Forrest Cotton Edkins, R. G. Cotton (Editor), S. Forrest (Editor)
 
 
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  ISBN: 0199636567
  Release Date: Jan 12, 1996


 
 
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Cotton, Richard G.H. (St Vincent's Hospital, Melbourne); Edkins, Edward (Joint Women's and Children's Hospital, Perth); Forrest, Sue (Murdoch Institute)

 
 
Table of Contents
 
List of Contributors
Abbreviations
Introduction1
References6
1Single-strand conformation polymorphism analysis7
2Single-stranded conformation polymorphism and heteroduplex analysis25
3Comprehensive mutation detection with denaturing gradient gel electrophoresis35
4Cleavage using RNase to detect mutations63
5Cleavage of mismatched bases using chemical reagents81
6Mutation detection using T4 endonuclease VII99
7Detection of mutations by hybridization with sequence-specific oligonucleotide probes113
8DNA detection and sequence distinction through oligonucleotide ligation131
9Detection of sequence variation using primer extension141
10Selective amplification of specific alleles161
11The protein truncation test (PTT)189
12Functional assay of the p53 tumour suppressor gene211
13Advances in direct DNA sequencing for mutation scanning223
List of Suppliers237
Index241


 
 
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