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"The artist is the confidant of nature, flowers carry on dialogues with him through the graceful bending of their stems and the harmoniously tinted nuances of their blossoms, Every flower has a cordial word which nature directs towards him."
- Auguste Rodin
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ZIN Product Number: 10042743
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| | | |  | | | Product Details
 Format: Paperback, 1st ed., 339 pages
Publisher: Oxford University Press
ISBN: 0199634947
Release Date: Jan 4, 1997
| |  | | | From The Publisher
Many investigations into cell structure and function require the isolation of a particular subcellular particle. Subcellular Fractionation covers the subject comprehensively, describing in detail the wide range of separation techniques and characterization procedures for all the major subcellular organelles: nuclei, mitochondria, chloroplasts, peroxisomes, and the membrane systems of the exocytic and endocytic pathways. Importantly, the text also describes the isolation of chromosomes, nucleoli and nucleoprotein complexes, and key procedures related to the analysis of these particles, such as the labelling of ligands, kinetic analysis of their internalization, and electron microscopy.
| | | | Annotation
Describes separation techniques & characterization procedures for all major subcellular organelles, etc
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| | | |  | | | | List of contributors | | | Abbreviations | | | 1 | Homogenization of tissues and cells | 1 | | 2 | Isolation of subcellular fractions | 31 | | 3 | Isolation and characterization of nuclei and nuclear subfractions | 71 | | 4 | Subcellular fractionation of mitochondria | 107 | | 5 | Isolation and characterization of peroxisomes | 143 | | 6 | Lysosomes and endocytosis | 169 | | 7 | The membranes of the secretory and exocytic pathways | 205 | | 8 | Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells | 243 | | 9 | Ribosomes and polysomes | 271 | | 10 | Electron microscopy of organelles | 303 | | A1 | Core list of suppliers | 329 | | Index | 333 |
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| | | | | | Keywords
Subcellular fractionation, Handbooks, manuals, etc, Laboratory manuals, Subcellular Fractions, methods, Science, Life Sciences - Cytology, Life Sciences - Biology - General, Handbooks, manuals, etc, methods, analysis, Laboratory manuals, Subcellular Fractions, Subcellular fractionation
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